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KMID : 1007520170260051371
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Food Science and Biotechnology
2017 Volume.26 No. 5 p.1371 ~ p.1377
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Anti-inflammatory and anti-genotoxic activity of branched chain amino acids (BCAA) in lipopolysaccharide (LPS) stimulated RAW 264.7 macrophages
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Lee Jae-Hoon
Park Eun-Ju
Jin Hyue-Ju
Lee Yun-Jeong
Choi Seung-Jun
Lee Gyu-Whan
Chang Pahn-Shick
Paik Hyun-Dong
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Abstract
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The purpose of this study was to evaluate the anti-inflammatory and anti-genotoxic activity of branched-chain amino acids (BCAAs) in lipopolysaccharide (LPS) stimulated RAW 264.7 macrophages. BCAAs inhibited LPS-induced NO production, with 100 mM leucine having the most pronounced effect, suppressing NO production by 81.15%. Valine and isoleucine also reduced NO production by 29.65 and 42.95%, respectively. Furthermore, BCAAs suppressed the inducible nitric oxide synthase mRNA expression. Additionally, BCAAs decreased the mRNA expression of interleukin-6 and cyclooxygenase-2 which are proinflammatory mediators. Anti-genotoxic activities of BCAAs were assessed using the alkaline comet assay and valine, isoleucine, and leucine significantly (p＜0.05) decreased tail length of DNA (damaged portion) to 254.8 ± 7.5, 235.6 ± 5.6, and 271.5 ± 19.9 lm compared than positive control H2O2 (434.3 ± 51.3 lm). These results suggest that BCAAs can be used in the pharmaceutical or functional food industries as anti-inflammatory agents or anti-cancer agents.
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KEYWORD
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Branched-chain amino acid, Anti-inflammatory activity, Anti-genotoxic activity, RAW 264.7 macrophage
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